Precautions for using Steam Sterilizer

Note: Completely remove the air in the Steam Sterilizer, so that the pot is completely filled with water vapor, and the sterilization can be complete. There are several different methods of autoclaving and venting, but the purpose is to remove the air, make the temperature of the pot evenly heated, and ensure the complete sterilization. The common method is: close the bleed valve, after power on, when the pressure rises to 0.05MPa, open the bleed valve to release air, and then close the bleed valve after the pressure gauge pointer returns to zero. After closing the valve and re-energizing, when the pressure gauge rises to 0.1MPa, start timing and maintain the pressure at 0.1～0.15MPa for 20 minutes.

After reaching the holding time, the power can be cut off. When the pressure drops to 0.5 MPa, the steam can be slowly released. Care should be taken not to reduce the pressure too fast, which may cause violent decompression boiling and overflow the liquid in the container. When the pressure drops to zero, the lid can be opened. Once placed for too long, the lid cannot be opened due to the negative pressure in the boiler. As long as the vent valve is opened, the atmospheric pressure enters, and the internal and external pressures are balanced, the lid will be easy to open.